Wednesday, September 22, 2010

DNA extraction and precipitation and necklacing

Introduction:
A) DNA Structure, was discovered by Watson and Crick using work from other scientists. The Structure is a double helix and the genes show the genetic plan of the building of each person. the DNA is located in the nucleus of each and every cell.
B)precipitate dna, which one can study and compare(test for diseases, traits, and C.S.I.)
C) Loosen cheek cells, take saline solutions to retrieve cheek cells(.9% saltwater)[isotonic] , add lysis buffer, dissolve cell wall/ membrane( soap/detergent) to dissolve phospholipids. The protease ( an enzyme that breaks down proteins) such as the DNAse. It speeds up the reaction. Put it in H2O bath, help break open cell membrane.

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